International Journal of Aquaculture Research and Development

Current Issue Volume No: 1 Issue No: 2

ISSN: 2691-6622
share this page

Review Article Open Access
  • Available online freely Peer Reviewed
  • Provisional

    Biotechnological application of Cyanobacteria in, Agriculture, Medicine and Environment

    Tefera Tadesse 1  

    1National Agricultural Biotechnology Research Center, Holotta, Ethiopia


    Cyanobacteria are considered as one of the important group of organisms having significant ecological, industrial, and biotechnological importance. Cyanobacteria have gained a lot of atten ion in recent years because of their potential applications in biotechnology. This review presents an overview of uses of cyanobacteria in industry agriculture, environment pharmaceutical and medicinal roles and to provide future prospects of the field of cyanobacteria biotechnology.

    Nowadays cyanobacteria have gained attention researchers because of their various potential applications such as food and feed pharmaceutical industries in medicine, in bioremediation, soil conditioning, as biopolymers, bio adhesives, bioenergy and biofertilizers. Due to presence of wide spectrum of bioactive compounds cyanobacteria has possesses antiviral, antibacterial, antifungal and anticancer activities. Several strains of cyanobacteria are also rich in food supplements. Further nitrogen fixing and soil conditioning capacity of cyanobacteria attracted researchers. Recent studies have also shown that cyanobacteria have capability to degrade environmental pollutants and are also being used as a promising source of alternative energy. Cyanobacteria has also its limitations through bloom production it influnces on the nutrient availability and usage of phytoplankton plants. This review is an effort to forward the valuable information about the qualities of cyanobacteria and their potential role in solving the agricultural and environmental problems for the future welfare of the planet.Thus more efforts should be made in search of more potential strains of cyanobacteria to ensure maximum production of the desired products.

    Author Contributions
    Received 16 Jun 2022; Accepted 09 Jul 2022; Published 12 Jul 2022;

    Academic Editor: Ghousia Begum, CSIR-Indian Institute of Chemical Technology, Ministry of Science & Technology, Government of India.

    Checked for plagiarism: Yes

    Review by: Single-blind

    Copyright ©  2022 Tefera Tadesse

    Creative Commons License     This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    Competing interests

    The authors have declared that no competing interests exist.


    Tefera Tadesse (2022) Biotechnological application of Cyanobacteria in, Agriculture, Medicine and Environment . International Journal of Aquaculture Research and Development - 1(2):34-43.

    Download as RIS, BibTeX, Text (Include abstract )

    DOI 10.14302/issn.2691-6622.ijar-22-4221


    Cyanobacteria are a group of aquatic organisms officially classified as bacteria, but they display characteristics of algae and bacteria. Cyanobacteria produce their own nutrients via photosynthesis. The color of the chlorophyll required for this process produces the coloration that has led to their common name, ‘blue green algae 1. Others explained cyanobacteria as common and natural aquatic organisms present in many surface waters. They are single celled microscopic bacteria and can be found in fresh, salt or brackish waters. Like plants, they use sunlight to make food and energy.

    In similar way 2 explained cyanobacteria or blue green algae as prokaryotic microalgae classified as a phylum of eubacteria. The blue green appearance of these bacteria is originated by the two pigments, chlorophyll a (green) and phycocyanin (blue) and which can survive in wide variety of environments. They are photoautotrophic in that they can harvest energy from the light source with their chlorophyll a pigment. Also, they consume CO2 to produce organic compounds plays a substantial task in the recycling of CO2 via their photosynthesis, which is similar to photosynthetic plants in oxygen generation. However, some species of cyanobacteria survive under mix trophic and heterotrophic conditions and as consortia with other microorganisms 3.

    Using water as an electron donor during photosynthesis resulting in the release of oxygen is another important characteristic of these tiny organisms. Furthermore, Number of findings reported that cyanobacteria have the ability to fix nitrogen. Cyanobacteria have been recognized as an opulent source of various bioactive compounds possessing anti bacterial, anti viral, anti fungal and anti cancer activities. They are also contributing positively in bioremediation and sustainable development of ecosystem. In view of the above, the objectives of the current review is to pointout overall contribution of cyanobacteria and their potential role in sustainable development of agriculture and ecosystem. This is also an effort to give the valuable information about multifunctional roll of cyanobacteria and their potential role in solving the agricultural and environmental problems for the future welfare of the planet.

    Cyanobacteria as Food and Feed Supplement

    The global population explosion has resulted in the need to look for alternative sustainable sources of food apart from conventional agricultural products. This has promoted interest in the use of functional foods to meet the nutritional demands of the growing human population. Micro organisms, especially cyanobacteria, are an untapped resource as their secondary metabolites have nutritional or therapeutic values. Commercial exploitation of cyanobacteria since the establishment of human civilization is owing to its different properties which make it a suitable source of functional foods. According to 4 many characteristics which make cyanobacteria a promising alternative for sustainable food production due to: high nutrient content of cyanobacteria, especially Spirulina, which is the most commercialized and cultivated cyanobacteria species. Its worldwide distribution, requirement of small amounts of water for growth including waste water, need for small land (unfertile & unsuitable for other crops), easily digestible and product stability over a wide pH and temperature range. According to some statistics findings of 5 1kg of Spirulina may replace 1000 kg of assorted fruits and vegetables in terms of nutritional value. The use of algae as an additive feed in aquaculture has also received a lot of attention due to the positive effect it has on weight gain. Quality of aqua feed is one of the most important criteria for the success of aquaculture. The popularity of microalgae particularly cyanobacteria as fish feed is increasing rapidly as suitable alternative source in modern aquaculture industry 6.

    Research findings from 7 reported that cyanobacteria have been assessed for their nutritional value into fish feed formulation which provides balanced nutrition and improved fish growth. According to8 the green algal species like Chlorella and Scenedesmus contains crude protein (40% to 50%), carbohydrate content (25% 60%) and a considerable amount of β carotene beneficial for the growth of fishes, and also use of Spirulina possessing protein as high as 40 70% is well documented as fish feed.

    There are numerous records of historical usage of cyanobacteria and microalgae in the human diet as it was reported by 9. Spirulina has been consumed by the local population in Chad after drying the biomass used for preparing dishes, such as meat and vegetable broth, and also sold in the local markets 10.

    As photosynthetic microorganisms, cyanobacteria harvest light as their energy source through a wide variety of photosynthetic capability that are rich in pigments have been reported to have beneficial health effects, e.g., providing micronutrients and macronutrients, aiding in digestion, etc. Among the most widely used species is the halo tolerant Spirulina spp due to high nutritional value and high digestibility and their richness in various nutrients and high protein content with additional health benefits as a source of antioxidants, coenzymes and vitamins 11.

    As it was reported by 12, some cyanobacteria like Nostoc strains can be eaten directly without performing purification since it contain 60% protein, rich in beta carotene, thiamine, riboflavin vitamin B12 and fibers thus plays an important role in nutritional composition of human diets.Similar findings reported by 13 that Spirulina, Anabaena and Nostoc are commonly used as a food source in different countries such as Mexico, Chile, and the Philippines) due to its popularity as a health and food supplement in the form of powder, tablets or capsules. Consumption of cyanobacteria results in several health benefits and considered traditionally as a “food for fitness”. Another type of cyanobacteria, Arthospira platensis with a rich source of β carotene and various biomolecules of nutraceutical value thus regarded as “food for the future” 14. Cyanobacteria produce carotenoids such as canthaxanthin, beta carotene, nostoxanthin, and zeaxanthin as food supplements, animal feed, food additives, and colorant. In addition to the above important factors Spirulina produce carotenoid such as the keto carotenoid, astaxanthin as powerful antioxidant which play a vital role in preventing damage in human cells through photo oxidation 15. He also reported that Astaxanthin obtained from Haematococcus pluvialis contains protease inhibitors that may be used to treat diseases, such as HIV (human immunodeficiency virus). Compounds and extracts with anti HIV activity have been reported but the amount of antiviral activity varies with the compound and extract.

    Medicinal Effects of Cyanobacteria

    The bioactive compounds available in cyanobacteria possess several medicinal benefits as it was suggested by 16 and have some specific applications such as their use in the formation of medicinal drugs.

    Other findings by 17 indicated that cyanobacteria have biochemical pathways that produce unique bioactive molecules with potential commercial and medical applications based on their anti fungal, antibiotic, antimicrobial, immunosuppressant, anti inflammatory, anticancer, antiviral, anti bacterial, anti coagulant, anti malarial, anti protozoan, anti tuberculosis, anti tumor and anti HIV (human immunodeficiency virus) and activities due to Cyanovirin N, a carbohydrate binding protein from Nostoc ellipsosporum 18

    In human clinical trials, supplementation with several species of Spirulina exhibited lipid lowering effects. In patients with diabetic type 2 diabetes its consumption showed significant reduction in ratios of total cholesterol and plasma lipids 19. Cyanobacteria compounds are found to target tubulin or actin filaments in eukaryotic cells, making them an attractive source of natural products as anti cancer agents due to molecules different types of anti microtubule agents have been in preclinical and/or clinical trials as potential anti cancer drugs 20.

    Findings by 21 reported that Arthrospira sp produces metabolites such as sulphated polysaccharides, which have anti viral, and anti cancer properties. They also form gamma linolenic acid (GLA) that is useful in the control of cholesterol levels, lowering blood pressure and protecting the cardiovascular system. These properties resulted from the availability of carotenoids, chlorophylls, phycocyanins, various amino acids and minerals 22. Human skin is one of the most complex body organs that functions as a physical barrier against water loss and environmental stressors including, pathogens, chemicals and physical agents. According to studies conducted by 23 cyanobacteria are rich sources of metabolites, which can be used to fight against skin related problem such as skin aging, fragility, laxity, enlarged pores, dryness, and wrinkles happen to skin texture as a result of chronic exposure to intrinsic and extrinsic destructive factors.

    24 also reported that Spirulina, Nostoc, Anabena and Oscillatoria strains are most commonly used in skin care produts for various skin conditions by acting as sunscreens, anti wrinkling agents, moisturizer or texture enhancing agents.

    Agricultural Applications of Cyanobacteria

    Nowadays agricultural practices heavily dependent on the application of synthetic fertilizers and pesticides, intensive tillage, and over irrigation, to meet the food requirement; nevertheless the effect on environmental, health, soil fertility, and increased cost of agricultural production.

    Similar reports suggested by 25 that sustainable agriculture practices as well as environmental quality besides eco friendly, low cost farming systems with the help of native microorganisms such as cyanobacteria (blue green algae) make the agro ecosystem more resilient, self regulating and also maintain the productivity and profitability 26.

    Some cyanobacteria have a capability have to solubilize soil phosphate since phosphorus (P) is the second important nutrient after nitrogen for plants and microorganisms,thus algae are particularly adapted to scavenge their environments for resources through structural changes, storage or increased resource utilization efficiency 27. Similarly 28 also pointed out thatdifferent adaptation methods of algae such as biochemical and physiological adjustments mechanisms enabled them to excrete substances to enhance nutrient availability, excrete extracellular phosphatases upon the onset of P limited conditions, store resources like P in excess of their immediate needs and change the pH of their surroundings, 27

    N fixers cyanobacteria increase the N content through nitrogen fixation in natural desert soils and released to the surrounding environment which make it available to plants or either released to the atmosphere in the form of N2O, or NO and HONO, which influence ozone and OH reactivity at the atmosphere 29.

    Cyanobacteria also influence the availability of P which is the second important nutrient to plants as they have the ability to transform non usable forms of inorganic P to a usable form through biological processes. Soil surface inoculated with different heterocystous and non heterocystous cyanobacteria has been reported to enhance total N, available N, and available P 30. In the field of agriculture, cyanobacteria have been mainly used as bio fertilizers due to their role as nutrient supplements. Also 31 reported that inoculation of soil with cyanobacteria specis such as Nostoc, Calothrix, Tolypothrix, and Scytonema have diverse N fixing potential in non water logged soils shown beneficial effects in terms of improvemen of quality of soil properties, enhancement in different plant growth such as wheat, maize, and lettuce as natural fertilizers 32. Cyanobacteria also provide a number of ecological roles that open the range for their application in agricultural systems from dry lands by providing promising extreme resistance to the abiotic stresses such as low rainfall, high radiation levels and long periods of drought.

    Cyanobacteria as Bio Controlling Agents

    Many research papers demonstrate the mechanisms of fungal and Oomycete growth inhibition by the activity of cyanobacteria extracts. Several extracts from, Anabaena spp., Fischerella sp., Nostoc spp., and Oscillatoria spp inhibited Aspergillus and mycelial growth due to methanol, acetone, diethyl ether, ethyl acetate, ethanol and methyl chloride extract depending on cyanobacteria 33 Other results of last investigations also give evidences about the defensive role of cyanobacteria and microalgeal secondary metabolites 34

    Cyanobacteria are known to produce metabolites with diverse biological activities such as anti bacterial, anti fungal and anti viral activities. Several reports 35, 36 have shown that the extracts of Nostoc species significantly inhibited the growth of phytopathogenic fungi because cyanobacteria produce biologically active compounds that have antibiotic and toxic activity against plant pathogens Other findings by 37 investigated the suppression effect of cyanobacteria species Nostoc endophytum and Nostoc muscurum against, the causal agent of soya bean root rot Rhizoctonia solani which confirms effect of cyanobacteria in biological control of wilt disease which may help to obtain a higher yield and good health in agriculture.

    Cyanobacteria as a Bio Energy

    The rapid growing population of the world continuously increases the global demand for fuel energy. The intensive use of fossil fuels worldwide leads to its depletion and will bring them close to the point of exhaustion due to unsustainable and nonrenewable nature. Thus, bio fuels are now a growing opportunity throughout the world as alternative to fossil fuels. Thus advantageous features of biofuels produced from microalgae biomass are renewability and a significantly smaller contribution to environmental pollution and global warming. The emission of greenhouse gases mainly CO2 from burning of fossil fuels are the main cause of global warming by releasing 29giga tons/year release of CO238 But bio fuels from algal have oxygen levels of 10 45% and very low levels of sulphur emission while petroleum based fuels have no oxygen levels with high sulphur emission. According to 39 cyanobacteria bio fuels are non polluting, locally available, accessible, sustainable and reliable fuel obtained from renewable sources. In addition to the above points microalgae algae based fuels are eco friendly, nontoxic and with strong potential of fixing global CO2.

    Cyanobacteria as Bio Remediating Agents

    Large quantities of synthetic pesticides, inorganic fertilizers and manure and/or bio solids amendments (usually containing pharmaceuticals and antibiotics residues) are regularly applied in agricultural land 40, 41. The translocation and losses of these contaminants usually happen through field runoff and drainage after irrigation or strong storm events leading to potential negative impacts against aquatic ecosystems (including aquifers) and eventually human health.

    Microalgae based systems have recently demonstrated to be very efficient in treating different types of wastewater, including domestic and agricultural runoff, removing not only nutrients such as nitrate, phosphate or ammonium, but also contaminants of emerging concern such as pharmaceuticals, pesticides or UV filters 42.

    The following cyanobacterial genera such as Anabaena, Arthrospira, Aphanothece, Chroococcus Fischerella, Lyngbya, Limnothrix, Nostoc, Oscillatoria and Phormidium were involved to remove various nutrients such as NO3-, NH3, PO43 and metals Cd, Co, Cr, Cu, Ni, Pb and Zn from different wastewaters (ground water, domestic and industrial sewage, synthetic, plating industry, urban, swine, agro industrial and animal wastewater 43.

    Cyanobacteria also play an important role in the biological treatment of wastewater, called “phyco remediation” by accumulating organic and inorganic toxic substances, as well as radioactive materials, in their cells and self purification of municipal, industrial, and agro industrial wastewater by developing several detoxifying mechanisms, including biosorption, bioaccumulation, biotransformation, bio mineralization, and in situ and/or ex situ biodegradation 44

    The same findings was reported by 45 that cyanobacteria can also be used as bioremediation agents to eliminate toxic wastes from contaminated sites including soil, water, wastewater, and sediments. In addition to the above pointes they degrade or detoxify many gaseous, solid, and liquid recalcitrant pollutants such as assimilate atmospheric nitrogen, remove heavy metals from aquatic ecosystems, and reduce the extra phosphate and nitrate in farmlands. Above all its usage as bioremediation agent was low cost, eco friendly nature, high efficacy, and public acceptance are the major advantages of using cyanobacteria for bioremediation 46

    Cyanobacteria for Bio-Plastics Production

    Cyanobacteria, have the potential to produce renewable biopolymers from natural resources such as, solar energy, water and CO2, reducing the need for fertile soils, fertilizers, herbicides and potable water for crop production. Among the cyanobacteria species Arthrospira (Spirulina), Synechococcus, and Synechocystis, are widely employed in biodegradable polymers such as PHAs production 47.

    Challenges of Cyanobacteria Cultures

    One of the challenges of cyanobacteria application is bioaccumulation process possibly affected by various factors, including the exposure route, duration, and concentration of Microcystin in their food resources, as well as bioaccumulation capacity and incomplete depuration after contact 48.

    Studies conducted by 49:50 reported that the toxin accumulates on the seafloor in snail, in fish tissues, in various mammal organs, including muscle, liver, kidney, heart, lung, spleen, gastrointestinal tract and gonads, consequently leading to potential damage.

    Similar research findings from 51:52 reported that Microcystin also affects vegetables and soils cereals, corn, peanuts, soybeans, and spices, among others during maturation, storage, and transportation. This toxin biomagnifies and persists in the medium of co occurrence, and poses a large potential ecological risk within the food chains which require urgent attention.

    Causes of Cyanobacteria Pollution

    Eutrophication is characterized by excessive algal growth due to the increased availability of one or more limiting growth factors needed for photosynthesis such as sunlight, carbondioxide, and nutrient fertilizers.

    Eutrophication occurs naturally over centuries as lakes age and are filled in with sediments 53. However, human activities have accelerated the rate and extent of eutrophication through both point source discharges and non point loadings of limiting nutrients, such as nitrogen and phosphorus, into aquatic ecosystems with dramatic consequences for drinking water sources, fisheries, and recreational water bodies. Similar findings by 54 reported that algal blooms to nutrient enrichment resulting from anthropogenic activities such as agriculture, industry, and sewage disposal and its consequences resulted in cultural eutrophication which include blooms of blue green algae (cyanobacteria), tainted drinking water supplies and degradation of recreational opportunities.

    Consequences of Cyanobacteria Blooms

    The most conspicuous effect of cultural eutrophication is the creation of dense blooms of noxious, foul smelling phytoplankton that reduce water clarity and harm water quality. Algal blooms limit light penetration, reducing growth and causing die-offs of plants in littoral zones while also lowering the success of predators that need light to pursue and catch prey 55. Furthermore, high rates of photosynthesis associated with eutrophication can deplete dissolved inorganic carbon and raise pH to extreme levels during the day. According to 56 elevated pH can in turn ‘blind' organisms that rely on perception of dissolved chemical cues for their survival by impairing their chemosensory abilities and eventually, algal blooms die, microbial decomposition severely depletes dissolved oxygen, creating a hypoxic or anoxic ‘dead zone' lacking sufficient oxygen to support most organisms.

    Strategies to Control Its Limitations

    Algal pollution led to water quality degradation associated with nutrient enrichment, eutrophication which continues to pose a serious threat to potable drinking water sources, fisheries, and recreational water bodies. It is mandatory to employ a variety of strategies to minimize the effects of cultural eutrophication, including; diversion of excess nutrients altering nutrient ratios, physical mixing, shading water bodies with opaque liners or water based stains, and application of potent algaecides and herbicides 57. Another alternative for improving water quality in nutrient rich lakes has been biomanipulation the alteration of a food web to restore ecosystem health as it was reported 58


    Cyanobacteria, known as “blue green algae”, are one of the oldest photosynthetic prokaryotes on planet earth, with the ability to live and flourish in a diverse range of environments from hot springs to underneath of ice pack in frozen lacks, and under the surfaces of rocks in deserts

    Cyanobacteria, like higher plants, are capable of converting light energy into chemical energy and generate O2. Due to interaction with other of microorganisms and their environment, cyanobacteria also produce a wide variety of secondary metabolites applied in feedstock for biofuel production, bioremediation agents to eliminate toxic wastes from contaminated sites including soil, water, wastewater, and sediments, bio fertilizers to improve soil fertility in agriculture, supplement for animal and aqua cultural feed, as well as human nutrition, and also in the pharmaceutical, food, and cosmetic industries. In recent year cyanobacteria have gained importance in various areas of research such as drug discovery, treatment of deadly disease such as HIV and cancer. A cyanobacteria also get attention due to their ability to fix atmospheric nitrogen and degrading pollutants and removing heavy metals by agriculturalist and environmentalist respectively. Cyanobacteria like Spirulina, Anabaena and Nostoc also are used to solve problem of food crisis and malnutrition in different contents. Thus the possibility of producing novel biopolymer blends, biofuel components, and pharmaceutical compounds that are capable of meeting the demands of a biotechnologically based society. Though cyanobacteria has various applications, conversely it has some limitations because of its bloom which in one or another way affect the ecosystem. To achieve these target great strides in the cyanobacteria production sector, a synergistic approach should be adopted by cyanobacteria-related companies so that more fruitful results will come out.


    1.Toxic Cyanobacteria in Water 1999: A guide to their public health consequences, monitoring and management, World Health Organization.
    2.Castenholz R, Rippka R, Herdman M, WIlmotte A. (2001) Subsection III. Formerly Oscillatoriales Elenkin. In:. Bergey’s Manual of Systematic Bacteriology, 2nd edition (Garrity, G., editor) 539-562.
    3.Subashchandrabose Ramakrishnan, Megharaj B, Venkateswarlu M, Naidu K, R. (2013) Mixotrophic cyanobacteria and microalgae as distinctive biological agents for organic pollutant degradation. , Environ. Int
    4.R B Dixit, M R Suseela. (2013) Cyanobacteria: potential candidates for drug discovery. , Antonie van Leeuwenhoek 103, 947-961.
    5.Singh S, Kate B, U C Banerjee. (2005) Bioactive compounds from cyanobacteria and microalgae: an overview. , Crit. Rev. Biotechnol 25, 73-95.
    6.E W Becker. (2007) Microalgae as a source of protein. , Biotechnol. Adv 25, 207-210.
    7.Velichkova K, Stoyanova S, Yordan S. (2015) The importance of microalgae for aquaculture. , Int. J Fish. Aquat. Stud 2(4), 81-84.
    8.Tartiel M M, Badwy J, Ibrahim E M, Zeinhom M M. (2008) Partial replacement of fishmeal with dried microalga (Chlorella sp and Scenedesmus sp.) in Nile Tilapia Oreachromismossambicus diets. 8th International Symposium on Tilapia in Aquaculture. Central Laboratory for Aquaculture Research, Agricultural Research Center, Ministry of Agriculture, Egypt 801-811.
    9.Tomaselli L, M R Palandri, M R Tredici. (1996) On the correct use of the Spirulina designation. , Algological Stud 83, 539-48.
    10.Abdulqader G, Barsanti L, M R Tredici. (2000) Harvest of Arthrospira platensis from Lake Kossorom (Chad) and its household usage among the Kanembu. , J. Appl. Phycol 12, 493-8.
    11.R N Krishnaraj, S V Babu, Ashokkumar B, Malliga P, Varalakshmi P. (2012) Antioxidant property of fresh and marine water cyanobacterial extracts in Swiss mice. , J. Biopestic 5, 250-254.
    12.Devillers J, J C Dore, Guyot M, Poroikov V, Gloriozova T et al. (2007) Prediction of biological activity profiles of cyanobacterial secondary metabolites. , SAR and QSAR in Environmental Research 18, 629-643.
    13.R M Abed, Dobretsov S, Sudesh K. (2009) Applications of cyanobacteria in biotechnology. , Journal of Applied Microbiology 106, 1-12.
    14.Mohan A, Misra N, Srivastav D, Umapathy D, Kumar S. (2014) Spirulina, the nature’s wonder: A review. , Lipids 5, 7-10.
    15.Singh R, Parihar P, Singh M, Bajguz A, Kumar J et al. (2017) Uncovering potential applications of cyanobacteria and algal metabolites in biology, agriculture and medicine: Current status and future prospects. Front. , Microbiol.;8 1-37.
    16.Romay C, Gonzalez R, Ledon N, Remirez D, Rimbau V. (2003) C-phycocyanin: A biliprotein with antioxidant, anti inflammatory and neuroprotective effects. , Current Protein and Peptide Science 4, 207-216.
    17.I C Dewi, Falaise C, Hellio C, Bourgougnon N, J L Mouget. (2018) . Anticancer, Antiviral, Antibacterial, and Antifungal Properties in Microalgae. Microalgae in Health and Disease Prevention 235-26.
    18.Luesch H, R E Moore, V J Paul, S L Mooberry, T H Corbett. (2001) Isolation of dolastatin 10 from the marine cyanobacterium Symploca species VP642 and total stereochemistry and biological evaluation of its analogue symplostatin 1. , Journal of Natural Products 64, 907-910.
    19.Lee E H, Park J E, Choi Y J, Huh K B, Kim W Y. (2008) A randomized study to establish the effects of Spirulina in type 2 diabetes mellitus patients. , Nutr Res Pract; 2, 295-300.
    20.Gerwick L T Tan, Sitachitta N. (2001) metabolites from marine cyanobacteria,” in The Alkaloids:. , San Diego, Calif, USA, Chemistry and Biology 57, 75-184.
    21.Sydney E B, Sturm W, Carvalho J C, Thomaz-Socclo V, Larroche C et al. (2010) Potential carbondioxide fixation by industrially important microalgae. , Bioresour. Technol 101, 5892-5896.
    22.M A Furmaniak, A E Misztak, Franczuk Wilmotte, Waleron A, Waleron M et al. (2017) Edible cyanobacterial genus Arthrospira: Actual state of the art in cultivation methods, genetics, and application in medicine. , Front. Microbiol 8, 1-21.
    23.M B Ariede, T M Candido, Jacome A L M, Velas¬co M V R, Carvalho J C M de et al. (2017) Cosmetic attributes of algae A review. , Algal research 25, 483-487.
    24.Derikvand P, C A Llewellyn, Purton S. (2017) metabolites as a source of sunscreens and moisturizers: A comparison with current synthet¬ic compounds European. , Journal of Phycology 52(1), 43-56.
    25.J S Singh, P J Strong. (2016) Biologically derived fertilizer: a multifaceted bio tool in methane mitigation. , Ecotoxicol. Environ. Saf 124, 267-276.
    26.Mason J. (2003) Sustainable Agriculture, 2nd Edn. , Collingwood, VIC: Landlinks Pre
    27.Singh N K.Dhar DW 2007.Nitrogen and phosphorous scavenging potential in microalgaeIndia. , J. Biotechnol.,6: 52-56.
    28.Healy F P. (1973) Characteristics of phosphorus deficiency in Anabaena. , J. Phycol 9, 383-394.
    29.Weber B, Wu D, Tamm A, Ruckteschler N, Rodríguez Caballero E. (2015) Biological soil crusts accelerate the nitrogen cycle through large NO and HONO emissions in drylands. Proceedings of the National Academy of Sciences 112(50), 15384.
    30.Wu Y, Rao B, Wu P, Liu Y.Li G 2013.Development of artificially induced biological soil crusts in fields and their effects on top soil. , Plant Soil 370, 115-124.
    31.Thajuddin N, Subramanian G. (2005) Cyanobacterial biodiversity and potential applicationsin biotechnology. , Current Science 89(1), 47-57.
    32.Sharma R, Khokhar M K, Jat R L.Khandelwal SK 2012.Role of algae and cyanobacteria in sustainable agriculture system. , Wudpecker Journal of Agricultural Research 1, 381-388.
    33.D A Marrez, Sultan Y Y. (2016) Antifungal activity of the cyanobacterium Microcystis aeruginosa against mycotoxigenic fungi. , J. Appl. Pharm. Sci 6, 191198.
    34.PANDY V D. (2015) Cyanobacterial natural products as antimicrobial agents. , Inter J Curr Microbiol Appl Sci 4(1), 310-317.
    35.Tassara C, Zaccaro M C, Storni M M, Palma M, Zulpa G. (2008) Biological control of lettuce white mold with cyanobacteria. , Int. J. Agri. Biol 10, 487-492.
    36.Zulpa G, M C Zaccaro, Boccazzi F, J L Parada, Storni M. (2006) Bioactivity of intra and extracellular substances from cyanobacteria and lactic acid bacteria on “wood blue stain” fungi. , Biol. Control 27, 345-348.
    37.ElH Mohamed, Osman M M, El Sheekh, Metwally A, Abd E A. (2011) Antagonistic Activity of Some Fungi and Cyanobacteria Species against Rhizoctonia solani. Ismail , International Journal of Plant Pathology 2, 101-114.
    38.Ho S H, Chen W M, Chang G. (2010) Scenedesmus obliquus CNW N as a potential candidate for CO2mitigation and biodiesel production. , Bioresour Technol 101(22), 8725-30.
    39.Paul Abishek M, Patel J, Prem Rajan A. (2014) Algae oil: a sustainable renewable fuel of future. Biotechnol Res Int. 272814.
    40.Gros M, Mas-Pla J, Boy-Roura M, Geli I, Domingo F et al. (2019) Veterinary pharmaceutical and antibiotics in manure and slurry and their fate in amended agricultural soils: Findings from an experimental field site (Baix Empordà. , NE Catalonia). Sci. Total Environ
    41.GMJ García, Gutiérrez R, Uggetti E, Matamoros V, Joan G et al. (2018) Use of full-scale hybrid horizontal tubular photobioreactors to process agricultural unoff. , Biosyst. Eng 166, 138-149.
    42.Li X, Cai F, Luan T, Lin L, Chen B. (2019) Pyrene metabolites by bacterium enhancing cell division of green alga Selenastrum capricornutum. , Sci. Tota Environ 689, 287-294.
    43.Mondal M, Halder G, Oinam G, Indrama T, O N Tiwari. (2019) Bioremediation of Organic and Inorganic Pollutants Using Microalgae. In New and Future Developments in Microbial Biotechology and Bioengineering; Elsevier: , Amsterdam, The Netherlands 223-235.
    44.Hall D O, Markov S A, Watanabe Y, Rao K K. (1995) The potential applications of cyanobacterial photosynthesis for clean technologies.Photosynth. Res.,46 159-16.
    45.Roeselers G, Loosdrecht M C M van, Muyzer G. (2008) Phototrophic biofilms and their potential applications. , J. Appl. Phycol 20, 227-235.
    46.Amadu A A, Qiu S, Ge S, GND Addico, Ameka G K et al. (2017) A review of biopolymer (Poly β hydroxybutyrate) synthesis in microbes cultivated on wastewater. Sci Total Environ.
    47.Xiang L, Y W Li, B L Liu, H M Zhao, Li H et al. (2019) High ecological and human health risks from microcystins in vegetable fields in southern China. , Environment International 133, 105142.
    48.Wang Q, Liu Y, Guo J, Lin S, Wang Y et al. (2019) Microcystin-LR induces angiodysplasia and vascular dysfunction through promoting cell apoptosis by the mitochondrial signaling pathway. , Chemosphere 218, 438-448.
    49.R S Quilliam, Niekerk M A van, D R Chadwick, Cross P, N Jones Hanley et al. (2015) Can macrophyte harvesting from eutrophic water close the loop on nutrient loss from agricultural land?. , Journal of Environmental Management 152, 210-217.
    50.Paldavičiene A, Zaiko A, Mazur-Marzec H, Razinkovas-Baziukas A. (2015) Bioaccumulation of microcystins in invasive bivalves: A case study from the boreal lagoon ecosystem. , Oceanologia 57(1), 93-101.
    51.Cao Q, Rediske R R, Yao L, Xie L. (2018) Effect of microcystins on root growth, oxidative response, and exudation of rice (Oryza sativa). Ecotoxicology and Environmental Safety. 149, 143-149.
    52.D W Schindler. (2006) Recent advances in the understanding and management of eutrophication. , Limnology and Oceanography 51, 356-363.
    53.W K Dodds. (2009) Eutrophication of U.S. freshwaters: analysis of potential economic damages. , Environmental Science and Technology 43, 12-19.
    54.Lehtiniemi M. (2005) Turbidity decreases anti predator behavior in pike larvae, Esox Lucius. , Environmental Biology of Fishes 73, 1-8.
    55.Huisman J. (2004) Changes in turbulent mixing shift competition for light between phytoplankton species. , Ecology 85, 2960-2970.
    56.Shapiro J. (1975) Biomanipulation: An ecosystem approach to lake restoration. In Water quality management through biological control 85-96.