Bacterial Broth Dilution

Method The bacterial broth dilution method is a lab technique used to assess the effectiveness of compounds against bacterial growth. This method involves adding a compound to a broth culture of bacteria and then measuring the amount of bacterial growth after incubation. By comparing the amount of bacterial growth in the presence of different concentrations of the compound, researchers can determine the minimum inhibitory concentration of the compound—the lowest concentration at which it halts bacterial growth. Knowing a compound’s minimum inhibitory concentration is important for researchers to be able to correctly evaluate its effectiveness against bacterial growth, which can be applied to making useful substances and applications, such as antibiotics or probiotics.